Journal: Pharmaceuticals
Article Title: Anti-Inflammatory Effects of GPR55 Agonists and Antagonists in LPS-Treated BV2 Microglial Cells
doi: 10.3390/ph17060674
Figure Lengend Snippet: Effects of KIT C ( A , B ), ML-193 ( C , D ), and O-1602 ( E , F ) on the expression ( A , C , E ) and release ( B , D , F ) of CXCL10 in LPS-stimulated BV2 cells. Cells were stimulated as described in the Materials and Methods section (4 h for qPCR, 24 h for ELISA). Values are presented as the mean ± SD of at least three independent experiments (N = 4 ( D ), N = 6 ( A – C ), N = 7 ( E ), N = 9 ( F )). Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc tests with ** p < 0.01, *** p < 0.001 and **** p < 0.0001 compared to LPS-stimulated cells.
Article Snippet: Commercially available ELISA kits were used following the manufacturer’s protocol for the determination of murine TNF-α, IL-6, CCL2, CCL3, CXCL2, and CXCL10 concentrations (cat. no.: DY410, DY406, DY479, DY450, DY452 and DY466, bio-techne/R&D Systems, Wiesbaden, Germany).
Techniques: Expressing, Enzyme-linked Immunosorbent Assay